Doc McStuffins

Jess Harnell

Chilly (voice), The Wicked King (voice), The Wicked King / Buddy (voice), The Dude / Rodriguez / Snowpeep (voice)

Lara Jill Miller

Lambie (voice), Mayella (voice), Snowpeep (voice), Hazel the Witch (voice)

Kimberly Brooks

Dr. Maisha McStuffins (voice), Surfer Girl (voice)

Gary Anthony Williams

Marcus McStuffins (voice), Butch (voice)

Loretta Devine

Nurse Hallie (voice), Hattie (voice)

Robbie Rist

Stuffy Filbert McStuffins (voice), Snowpeep (voice)

Kiara Muhammad

Doc McStuffins (voice)

Jaden Betts

Donny McStuffins (voice)

Laraine Newman

Professor Hootsburgh (voice), Southwest Sal (voice), Aurora (voice), Aurora / Professor Hootsburgh (voice), Southwest Sal / Snowpeep (voice), Trina Triceratops (voice), Trixie (voice)

Jeffrey Nicholas Brown

Bronty (voice), Ben (voice), Otis / Tumbleweed (voice)

Rob Paulsen

Sir Kirby (voice)

Grey DeLisle

Dress-Up Daisy (voice), Bouncy Babies (voice)