Your Friendly Neighborhood Spider-Man

Kari Wahlgren

May Parker (voice), Roxanna Volkov (voice), Mrs. Eberhardt (voice)

Zeno Robinson

Harry Osborn (voice), Mr. Campbell (voice)

Hudson Thames

Peter Parker / Spider-Man (voice)

Colman Domingo

Norman Osborn (voice)

Eugene Byrd

Lonnie Lincoln (voice)

Grace Song

Nico Minoru (voice)

Cathy Ang

Pearl Pangan (voice)

Erica Luttrell

Asha (voice), Emma (voice), Mrs. Lincoln (voice), Emma / Mrs. Lincoln (voice)

Roger Craig Smith

Coach Grayfield (voice), Dimitri Smerdyakov (voice), Dmitri Smerdyakov (voice), James 'Speed Demon' Sanders (voice), Dmitri Smerdyakov / Chameleon (voice)

Leilani Barrett

Big Donovan (voice), Big Donavan (voice)

Zehra Fazal

Dr. Connors (voice), Dr. Connors (voice), Susan O'Harra (voice), Hot Rod (voice)

Paul F. Tompkins

Bentley Wittman (voice)